Analysis of Bacillus subtilis 168 prophage-associated lytic enzymes; identification and characterization of CWLA-related prophage proteins
نویسندگان
چکیده
منابع مشابه
Characterization of PBSX, a defective prophage of Bacillus subtilis.
PBSX, a defective Bacillus subtilis prophage, maps to the metA-metC region of the chromosome. DNA (33 kilobases) from this region of the chromosome was cloned and analyzed by insertional mutagenesis with the integrating plasmid pWD3. This plasmid had a promoterless alpha-amylase gene (amyL) that provided information on the direction and level of transcription at the site of integration. Transcr...
متن کاملPhysical and genetic mapping of the SPO2 prophage on the chromosome of Bacillus subtilis 168.
It has been found by density transfer and genetic mapping experiments that prophage SPO2 is linked to the antibiotic resistance marker ery-1 in Bacillus subtilis 168.
متن کاملLysis genes of the Bacillus subtilis defective prophage PBSX.
Four genes identified within the late operon of PBSX show characteristics expected of a host cell lysis system; they are xepA, encoding an exported protein; xhlA, encoding a putative membrane-associated protein; xhlB, encoding a putative holin; and xlyA, encoding a putative endolysin. In this work, we have assessed the contribution of each gene to host cell lysis by expressing the four genes in...
متن کاملThe N - acetylmuramoyl - L - alanine amidase encoded by the Bacillus subtilis 168 prophage SPP Alexandre Regamey and Dimitri Karamata
Heat shock of Bacillus subfi/is CU1147, a strain lysogenic for SPpc2, a prophage with a thermosensitive repressor, results in phage induction and subsequent cell lysis. Cloning in Escherichia coli and sequencing of a DNA fragment of prophage SPp led to the identification of blyA, the gene encoding a 367 amino acid polypetide with a molecular mass of 39.6 kDa. Purified BlyA obtained from the E. ...
متن کاملInduction of prophage SPO2 in Bacillus subtilis: prophage excision in the absence of bacterial or bacteriophage DNA synthesis.
Bacillus subtilis lysogenic for SPO2 wild type was induced under conditions preventing synthesis of both bacterial and phage DNA. The infectivity of phage DNA in transfection is strongly decreased under these conditions, whereas the activity of single phage genes as measured by marker rescue with superinfecting phage is unaffected. DNA from induced cells was sedimented in neutral sucrose gradie...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1993
ISSN: 0022-1287
DOI: 10.1099/00221287-139-12-3177